The FluoProdige Protein Quantification Assay Kit presents a complete fluorometric assay for protein and peptide quantification.FluoProdige facilitates and improves protein quantification by providing sensitivity and convenience when compared to classic colorimetric measurements such as Lowry or BCA.
FluoProdige Assay Kit main benefits:
- Accurate & highly sensitive
Detect as little as 40 ng/mL - Large linear detection range: Over 3-orders of magnitude
- Simple & Fast
Protocol: 15 min - Pic of signal within 30 min - Signal duration: up to 4 hours
- Safe & Biodegradable natural product: non toxic, heavy metal free
- Compatible with many buffers
Robust to interfering compounds such as DNA, Detergents, DTT and Urea
This assay kit uses a stable analogue to epicocconone molecule that reversibly binds to lysine, arginine and histidine residues in proteins and peptides to yield an intense red-fluorescent product. The fluorescent signal (~518/605) is directly proportional to protein amount among a wide range of protein concentration, rendering this kit highly sensitive. When bound to proteins, excitation and emission signals are 518/605 nm respectively.
Kit contains:
- FluoProdige fluorescent Dye (10 mL) / Dilution Buffer (100 mL) / BSA (500µg) lyoph
Storage: Upon receipt, store all reagent tubes at 4°C (up to 6 months). For long term storage, store the dye and BSA at -20°C.
Shipping condition: The kit is shipped at RT.
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