BCA Protein Assay kit (BCA-PAK) is useful for colorimetric detection and quantification of total protein content even in the presence of detergents.

This kit is based on the reduction of Cu²⁺ to Cu¹⁺ by protein in alkaline solution; monovalent copper ions produced are detected in a concentration-dependent manner. Bicinchoninic acid (BCA) chelates with the reduced copper Cu¹⁺ and forms a water-soluble purple reaction complex that exhibits a strong absorbance at 562 nm. Absorbance is linear over a wide range of protein concentrations between 25-2000 µg/mL.        In general, protein concentrations are estimated with reference to a commonly used protein standard; the kit also includes Bovine Serum Albumin (BSA) at 1.5 mg/mL as a protein standard for a convenient preparation of protein concentration standard curves. 

Kit contains:

• BCA Reagent A (500mL) / Copper Solution B (15mL) / BSA 5x1mL - 1500µg/mL

Storage: Store at 4°C

Shipping condition: The kit is shipped at RT.

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