OZ Biosciences SEAP Assay Kit (Secreted Alkaline Phosphatase) is a colorimetric assay for sensitive quantification of SEAP in culture medium from transfected cells or tissues. Alkaline phosphatase cleaves the colourless 4-nitrophenylphosphate (pNPP) substrate to give a yellow p-nitrophenolate product in alkaline solutions. It is commonly used as a reporter gene expression and can be accurately quantified. It is a truncated form of placental alkaline phosphatase (GPI-anchored protein).
The kit contains sufficient reagents to perform 500 assays in 96-well plate format.
SEAP assay kit main features:
Designed to detect SEAP reporter gene expression
Fast and easy analysis
Colorimetric – measure with a standard spectrophotometer or an ELISA reader (405 nm)
Multiple samples can be analyzed in low volumes
Convenient and economical packaging: no tablet, no powder
Sizes:pNPP-containing Assay Buffer component A: 10 x 1 mL
Reaction Buffer #1 component B: 125 mL
Reaction Buffer #2 component C: 125 µL
Dilution Buffer component D: 50 mL
Storage: -20°C for component A / +4°C for components B, C and D
Shipping Conditions: Room Temperature
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