Plasmids and viral vectors are traditionally used in CRISPR genome editing to express the required proteins. However, there is a risk to use DNA instead of mRNA: Double stranded DNA breaks catalyze insertion of DNA at the cut site. At some substantial frequency, the protein expression vectors can integrate, which can lead to continuous expression of the nuclease or a previously silent sequence.
Now mRNA is becoming a powerful tool in genome editing. With no risk of insertional mutagenesis, mRNA are used to transiently express the required proteins. Wild-type Cas9 mRNA is a stabilized non-immunogenic messenger RNA (mRNA) that has been designed to produce high expression level of wild-type Cas9 protein. It creates a double stranded break at a target site delineated by RNA guide sequences.
This mRNA is produced by in vitro transcription, stabilized at the 5’ end by modified nucleotides capping and contain a poly(A) tail at the 3’ end. mRNACas9 is optimized to yield improved stability and performance and to lower the cellular innate immunity response.
mRNA transfection provides several advantages :
- No need of nuclear uptake - protein expression directly in cytoplasm
- Faster protein expression than DNA transfection
- No genomic integration
- Perfect for transfecting slowing or non-dividing cells
- Protein expression in a total promoter-independent manner
- Transient transfection: mRNA based expression of proteins sustains for a limited time
Size: 20 µg or 100µg
For bulk, please contact us: order@ozbiosciences.com
Storage: -80°C
mRNAs ARE SHIPPED ON DRY ICE
Dry ice fee included in shipping cost
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