Reporter genes are commonly used in cell biology research. 

OZ Biosciences offers reporter mRNAs that can be used as controls to study transfection and expression in mammalian cells using a variety of assays. They mimic fully processed mature mRNA.

These mRNAs are produced by in vitro transcription and stabilized at the 5’ end by modified nucleotides capping (Cap1). These mature mRNA contain a poly(A) tail at the 3’ end and are also optimized to yield improved stability and performance.

We also offer 5moU modified mRNAs -- #MRNA10 (Tomato), #MRNA11 (GFP), #MRNA12 (F-Luc), #MRNA13 (mCherry) and #MRNA14 (β-gal).

We recommend using our transfection reagents RmesFect or RmesFect Stem in order to reach high transfection efficiency and optimum protein expression level.

mRNA transfection provides several advantages :

• No need of nuclear uptake - protein expression directly in cytoplasm

• Faster protein expression than DNA transfection

• No genomic integration

• Protein expression directly related to mRNA quantity

• Perfect for transfecting slowing or non-dividing cells

• Protein expression in a total promoter-independent manner

• Transient transfection: mRNA based expression of proteins sustains for a limited time

• GFP mRNA has been designed to produce high expression level of Green Fluorescent Protein. It is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm. 

• F-Luc mRNA has been designed to produce high expression level of FireFly Luciferase. It is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.
• Tomato mRNA has been designed to produce high expression level of Orange Fluorescent Protein. The detection can be done by fluorescent or confocal microscopy. The produced Tomato has an excitation maximum at 551-557 nm and emission maximum at 579-583 nm. Tomato expression level can also be monitored by fluorescence-activated cell sorter analysis (FACS).

Size: 20 µg,100µg and 1 mg

For bulk, please contact us: order@ozbiosciences.com

Storage: -80°C

mRNAs ARE SHIPPED ON DRY ICE

Dry ice fee included in shipping cost

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The following items are unmodified mRNAs -- if you need 5moU modified mRNAs, please visit this page.